Choose from over 8 million predesigned assays covering 23 species. If we don’t offer a predesigned assay for your target, custom TaqMan® probes and primers can be manufactured for your target …

TaqMan probes are hydrolysis probes that are designed to increase the specificity of quantitative PCR.

Jun 13, 2024 · TaqMan probes are used to quantify the amount of viral RNA or DNA in clinical samples, such as those from patients with HIV or Hepatitis. Monitoring viral load is essential for diagnosing …

Jul 24, 2025 · The TaqMan probe is a third short DNA strand that binds to a specific sequence between the primer binding sites. This probe is modified with two fluorescent molecules: a reporter dye at one …

TaqMan probes are linear probes that are dual-labeled with a reporter dye and a quencher dye. During the extension phase of the PCR, the TaqMan probe hybridizes to its target.

When the polymerase reaches a TaqMan probe, its endogenous 5' nuclease activity cleaves the probe, separating the dye from the quencher. With each cycle of PCR, more dye molecules are released, …

In this article, we delve into the science behind the TaqMan assay, break down its mechanism, and explain how fluorescent probe cleavage enables gene expression measurement.

TaqMan assays are a type of real-time polymerase chain reaction (PCR) that utilize fluorescent probes to monitor the amplification of specific DNA sequences. Named after the Thermo Fisher Scientific …

Nov 28, 2025 · The developed TaqMan probe-based real-time PCR assays were tested for sensitivity, specificity, and applicability in chevon meat by artificial spiking of target pathogens. The study …

Apr 29, 2025 · TaqMan probes have become essential in modern quantitative PCR (qPCR) methods, offering high specificity and sensitivity for nucleic acid detection. This technology is crucial in …