This led to many research groups using a control called the Isotype control. The concept of this control is that an antibody targeting a protein not on the surface of the target cells, has the same isotype (both …

What are Isotype Controls? In flow cytometry, background levels of staining can be a problem especially with rare populations, cells with low expression levels and when building multicolor panels. Isotype …

Jun 6, 2024 · Isotype controls are negative controls that allow you to determine what level of non-specific binding you have in your sample. The isotype antibody will be virtually identical to the …

There are some situations in the clinical flow lab where isotypic/isoclonic controls may continue to be appropriate such as: intracellular staining, quantitative applications, rare event analysis, etc.

An isotype control is an antibody raised against an antigen not present on or in the analyzed cell type. Isotype controls determine the level of background fluorescence caused by non-specific antibody …

The selection of an appropriate isotype control is essential for every flow cytometry experiment. Isotype control Abs that have no relevant specificity help to distinguish non-specific "background" staining …

Oct 16, 2024 · What is Isotype Control? An isotype control (Isotype Control) refers to an antibody that shares the same species origin, subtype, fluorescent label, dosage, and concentration as the flow …

Aug 26, 2025 · An isotype control is a type of antibody used as a negative control in flow cytometry experiments to address the issue of non-specific binding. This control antibody has no known …

In flow cytometry, having the right controls is every bit as important as staining your samples with the test antibodies. From compensation beads to autofluorescence and Fc blocking, our new webpage …

Mar 16, 2021 · Antibody isotype controls are essential negative controls for in vitro and in vivo studies and are used extensively in applications such as flow cytometry, immunohistochemistry, …