Since its introduction 6 years ago, the Gibson Assembly method has been widely adopted as a preferred cloning method. Here we describe an application of Gibson Assembly beyond routine cloning: ...

Gibson Assembly Cloning is an elegant and robust seamless cloning methodology that has been widely adopted by the scientific community and enables the assembly of multiple DNA fragments of varying ...

The Gibson Assembly® method is an established DNA assembly reaction that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, single-tube, isothermal reaction (Invitrogen™ ...

Large-scale DNA assembly stands as a foundational pillar in synthetic biology, empowering genomic engineering capabilities from targeted modifications to de novo genome synthesis. This technique ...